incubated hatching started within 1618 hours. Franciscan A. newborn (nauplii are) used within 10 hours of incubation. Nauplii are placed on artificial lighting nauplii abundance of water has been removed for biological analysis at the end nearest the light of their own culture vessel separate the skins and the rest of the cyst and concentrated to an appropriate concentration. Seawater contains about 25 400 ㎕ this added to the wells (mean 25.2, N = 286, SD 8.6) for the larvae 48 well plate, which was used immediately in the bioassay. A. vera juice was serially diluted in deionized water. Dilute the juice was transferred to the rules of toxins ranging up to 400 ㎕ and 25 + or - even low 1 artificial lighting (500 lux) in culture] C. Negative control (400 ㎕ water) it has been executed in the less for each dish. All concentrations treatment was carried out in at least. Check regularly and so was not dead. So that any movement of the parts is not observed within 10 seconds was considered dead nauplii. After 72 hours, all nauplii were counted to determine the total expenditure per well. In each treatment [CL50] 95% confidence limits were calculated using probit analysis [24].

antioxidant effect on the toxicity induced Aloe Vera

antioxidant coprocessing

To determine the ability of the antioxidant to block the toxic effects of Aloe vera juice, vitamin C, freshly prepared from deionized water in g / ㎖ solution 400 [micro]. Vitamin E and Trolox were [TM were each dissolved in 60% methanol, and 400 micro] diluted in distilled water to a concentration of g / ㎖. Aloe Vera juice 24% solution was diluted with deionized water.

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It is obtained from aloe vera juice Aloe Wellness Pty Ltd, Australia, and stored at 4 [degrees] C until use. A. juice Aloe Vera serial dilution in distilled water for use in the bioassay franciscana. Vitamin E (alpha-tocopherol, Sigma, purity> 96%) was dissolved in 60% methanol to provide ㎎ / ㎖ store 10. This mother is diluted in distilled water for use in the bioassay series. Trolox [TM (Sigma, purity> 97%) prepared in 1.5 ㎎ / social ㎖ 60% methanol and then diluted in distilled water for use in the bioassay. Vitamin C - dissolve the (L-ascorbic acid, grade AR, supply of chemicals) which was diluted with deionized water. Emodin (Sigma,> 95% purity) in aloe was prepared by dissolving in distilled water, in order to serially diluted in deionized water in g / ㎖ to provide a concentration of 500 micro]. All reagents were prepared fresh before use.

2. Biological toxins reference test

potassium dichromate - prepared in (AR grade, supply of chemicals) to a solution of 1.6 ㎎ / ㎖ in distilled water, and diluted with artificial seawater for use in the bioassay of Artemia nauplii series . Mevinphos (2- methoxy-1-methylvinyl dimethyl phosphate) mixture of cis (76.6%) and trans (23.0%) Sigma-isomer was prepared by 4 ㎎ / ㎖ of distilled water to obtain stock from Aldrich. Stock was diluted in artificial seawater for use in the bioassay series.

3. bioassay

And tested using a modified form of the development of Artemia nauplii Meyer lethal toxicity analysis. The selection of plants active ingredients [23]. Artemia cysts Franciscan Kellogg North America (Great Salt Lake, Utah harvest) LLC, obtained from the United States. leaf salt synthetic sea water, was prepared using azoo (Note), migukreul. water solution to 34 g / ℓ of distilled water were prepared before use. A. franciscana cysts in the degree 2g 25] C, cells were grown in a continuous aeration, 1 2000 liters under artificial light water Lux synthetic sea.

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A warm divine original aloe vera drink
It is essential for the establishment of a warm divine original aloe vera drink to entertain buyer categories success motivated buyers. It has been done to overcome barriers in order to proceed buy a flow of good neighborliness and clear buyer confusion and proliferation Range - Hot drinks now have a justified reputation display clearly influential and active store.

There are still important to support and educate buyers. This increase signal system by the buyer for easy navigation, for example, be achieved by altering the layout inside the store. Coffee noted the need to go to the direction of the machine, and much more destructive testing of the selection of merchandising Ford’s participation in the category of buyer. This business can reduce its dependence on volume sales promotion.

Type of packaging, materials, and including the 2015 forecast range of divine original aloe vera drink consumption data by individual package

Reasons to buy

* Produced in 2015 by Canadean forecast drink beverage packaging market in the Philippines to provide a thorough understanding of packaging trends. Category, type of packaging, packaging material and individual pack size (historic and forecast 2015) includes data divine original aloe vera drink consumption. When it packaged as part of the manufacturing process for calculating the raw data that useful requirements million units was measured. data divine original aloe vera drink containers is available to buy as a global, regional or country that allows easy comparison of market structure and trends between different countries and regions level

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Important supplier of delectable mango aloe vera drink
Delectable mango aloe vera drink pods are the key segment to take advantage of this trend actors. NESCAFÉ Dolce Gusto [R] is the same cafeteria offers a variety of quality coffee: Coffee Americano, cappuccino, latte macchiato and espresso category, and has contributed to the steady growth trend seen in the last year.


important supplier of delectable mango aloe vera drink machines is supporting this trend through television and proactive disclosure strategy in the last year. Continued product innovation and NPD helped launch this category with the Ford development.

The recruitment of young people

The key to the future maintenance of the priorities of the category of hot drinks and attract consumers in the future.

Only younger consumers (under 34 years) accounted for 12.3% (5) of current expenditure hot drink. In response, the ratings are convenient and appealing to the attention of buyers of these products as NESCAFÉ ORIGINAL 3IN1 [R], the paper provides a bag of coffee and white sugar. NESCAFÉ ORIGINAL overcome any challenge with writing it is one of the main obstacles to the sweet taste profile of 3IN1 young audience. Communication and participation are relevant to them, the results speak for themselves (5): 4.3 million (6) strong sales index [pounds sterling] among younger buyers throughout the current year and moving. 7

VALUE optimization optimization PRIORIT value is more important than ever. promotion force continues to grow; The soluble delectable mango aloe vera drink sold in transactions has increased significantly from 56% to 63% (8) sold in the trading volume over the standard car has increased from 56% to 59% (8). The more than 85% of coffee sales in the key driven by the same reference this promotion.

Provide the value and enjoyment of the buyer through the promotion, marketing lever legitimate, but it is necessary to introduce new consumers to the category to drive growth by creating a category if the drink new sustainable equilibrium. For example, soluble delectable mango aloe vera drink, the well and performing a fill type now [pound] or more values ​​31.3m (4). NESCAFÉ COLD MIX [R] is the fastest growing SKU of any 150g refill pack Refill (9).

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In the current demand for quality appetizing strawberry aloe vera drink at home
During the past 12 months will continue to grow ...

hot drink category growth trend of + 3.7% (1) 1.55bn the previous 12 months [pounds sterling, and continues to grow. This trend is the low rate of increase of 59% in the importance of responding throat’- hot category category -reinforcing (compared to 52% last year) of hot appetizing strawberry aloe vera drink consumers the option of consuming their drinks.

category segments and regions successful hot coffee eumryoyi Ford grown in the value of the previous amount. Ford (2), double-digit increase in value (+ 49%) and sustainable prosperity for grinding roasted appetizing strawberry aloe vera drink sales 11.4% (1).

This is to strengthen these current availability of these and other sectors such as coffee, quality certification that fits most consumer trends can learn from success.

Tea and coffee is the value of + 4.3%, respectively (1) + 1.3% (1) The standard enrollment growth in the industry daily. Hot chocolate can see the growth of the growth and prosperity of special vehicles and + 7.3% + 3.8% over the last year (1).

The key to maintaining the momentum to keep the scope of the hot drink, however, was good in recent years. PND quality and looks hot main categories in the right investment and the future.

In the current demand for quality coffee at home

Hot drinks outside the home continued to influence the tastes and habits of consumers. Since availability ’microground’ and the arrival of Starbucks VIA coffee Kenco Millicano a brand segment, which began in 2010, where guests can enjoy a high-quality appetizing strawberry aloe vera drink at home in the comfort of your own home. The result is the test of success: the segment is already worth [pounds] 6 (3) to obtain a strong penetration of the rapid 8.4% of households in the UK (4).

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