The toxicity induced Aloe Vera

incubated hatching started within 1618 hours. Franciscan A. newborn (nauplii are) used within 10 hours of incubation. Nauplii are placed on artificial lighting nauplii abundance of water has been removed for biological analysis at the end nearest the light of their own culture vessel separate the skins and the rest of the cyst and concentrated to an appropriate concentration. Seawater contains about 25 400 ㎕ this added to the wells (mean 25.2, N = 286, SD 8.6) for the larvae 48 well plate, which was used immediately in the bioassay. A. vera juice was serially diluted in deionized water. Dilute the juice was transferred to the rules of toxins ranging up to 400 ㎕ and 25 + or - even low 1 artificial lighting (500 lux) in culture] C. Negative control (400 ㎕ water) it has been executed in the less for each dish. All concentrations treatment was carried out in at least. Check regularly and so was not dead. So that any movement of the parts is not observed within 10 seconds was considered dead nauplii. After 72 hours, all nauplii were counted to determine the total expenditure per well. In each treatment [CL50] 95% confidence limits were calculated using probit analysis [24].

antioxidant effect on the toxicity induced Aloe Vera

antioxidant coprocessing

To determine the ability of the antioxidant to block the toxic effects of Aloe vera juice, vitamin C, freshly prepared from deionized water in g / ㎖ solution 400 [micro]. Vitamin E and Trolox were [TM were each dissolved in 60% methanol, and 400 micro] diluted in distilled water to a concentration of g / ㎖. Aloe Vera juice 24% solution was diluted with deionized water.

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