It is obtained from aloe vera juice Aloe Wellness Pty Ltd, Australia, and stored at 4 [degrees] C until use. A. juice Aloe Vera serial dilution in distilled water for use in the bioassay franciscana. Vitamin E (alpha-tocopherol, Sigma, purity> 96%) was dissolved in 60% methanol to provide ㎎ / ㎖ store 10. This mother is diluted in distilled water for use in the bioassay series. Trolox [TM (Sigma, purity> 97%) prepared in 1.5 ㎎ / social ㎖ 60% methanol and then diluted in distilled water for use in the bioassay. Vitamin C - dissolve the (L-ascorbic acid, grade AR, supply of chemicals) which was diluted with deionized water. Emodin (Sigma,> 95% purity) in aloe was prepared by dissolving in distilled water, in order to serially diluted in deionized water in g / ㎖ to provide a concentration of 500 micro]. All reagents were prepared fresh before use.

2. Biological toxins reference test

potassium dichromate - prepared in (AR grade, supply of chemicals) to a solution of 1.6 ㎎ / ㎖ in distilled water, and diluted with artificial seawater for use in the bioassay of Artemia nauplii series . Mevinphos (2- methoxy-1-methylvinyl dimethyl phosphate) mixture of cis (76.6%) and trans (23.0%) Sigma-isomer was prepared by 4 ㎎ / ㎖ of distilled water to obtain stock from Aldrich. Stock was diluted in artificial seawater for use in the bioassay series.

3. bioassay

And tested using a modified form of the development of Artemia nauplii Meyer lethal toxicity analysis. The selection of plants active ingredients [23]. Artemia cysts Franciscan Kellogg North America (Great Salt Lake, Utah harvest) LLC, obtained from the United States. leaf salt synthetic sea water, was prepared using azoo (Note), migukreul. water solution to 34 g / ℓ of distilled water were prepared before use. A. franciscana cysts in the degree 2g 25] C, cells were grown in a continuous aeration, 1 2000 liters under artificial light water Lux synthetic sea.

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